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. 1993;37(3):229-32.
doi: 10.1111/j.1348-0421.1993.tb03204.x.

In vitro cultivation of Borrelia duttonii on cultures of SflEp cells

H Konishi  1 , M G Morshed, H Akitomi, T Nakazawa
Affiliations
Free article

In vitro cultivation of Borrelia duttonii on cultures of SflEp cells

H Konishi et al. Microbiol Immunol. 1993.
Free article

Abstract

Borrelia duttonii strain 406K, a causative agent of relapsing fever, could not be cultivated in vitro in currently available media for borreliae. We have developed an in vitro cultivation system by using SflEp cell cultures. The average increases of the number of borreliae, when inoculated with 1.0 x 10(5) organisms per ml from infected mice, were 23-fold and 150-fold in the primary culture and the 3rd subculture, respectively. Even a single borrelia could propagate in this cultivation system. This system will be useful for immunological and physiological studies on uncultivable Borrelia strains.

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