doi: 10.1007/BF02919009.
Gene manipulation by protoplast fusion and penicillin production by Penicillium chrysogenum
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- PMID: 8323265
- DOI: 10.1007/BF02919009
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Gene manipulation by protoplast fusion and penicillin production by Penicillium chrysogenum
Appl Biochem Biotechnol.
1993 Spring.
Abstract
Hybrids have been obtained by protoplast fusion of nitrate-nonutilizing cnx- and acetate-nonutilizing fac- mutants of Penicillium chrysogenum strains 4/95 and 26/818, respectively. Induced haplodization of the hybrids allowed the recovery of stable segregants, which were screened for penicillin production. The penicillin-producing segregants showed a wide range of titers that reached for a certain mutant to 290-390% increase above the parent strains.
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