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. 1993 May-Jun;4(3):212-8.
doi: 10.1021/bc00021a005.

p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling

M E Annunziato  1 , U S Patel, M Ranade, P S Palumbo
Affiliations

p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling

M E Annunziato et al. Bioconjug Chem. 1993 May-Jun.

Abstract

p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.

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