Long range restriction mapping of 13q14.3 focused on the Wilson disease region

Abstract

The Wilson disease locus (WND) has been mapped by multipoint linkage analysis to a region within 13q14.3 that is flanked proximally by marker D13S31 and distally by marker D13S59 at distances of 0.4 and 1.2 cM, respectively. Long range restriction maps were constructed around these two markers to provide a framework for the detailed physical analysis that will be necessary for the cloning of the gene. The maps, together spanning over 4 Mb, include five newly isolated markers (D13S110, D13S113, D13S194, D13F71S1, and D13S196) and two established markers (D13S56 and D13S25) that were previously localized to 13q14.2-q14.3 using a hybrid panel. Markers D13F71S1 and D13S196 map to the region between D13S31 and D13S59 and will therefore be useful for the isolation of YACs for further molecular studies on Wilson disease.