Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jul 1;90(13):6213-7.
doi: 10.1073/pnas.90.13.6213.

Complex formation between RAS and RAF and other protein kinases

L Van Aelst  1 , M Barr, S Marcus, A Polverino, M Wigler
Affiliations

Complex formation between RAS and RAF and other protein kinases

L Van Aelst et al. Proc Natl Acad Sci U S A. .

Abstract

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

PubMed Disclaimer

References

    1. Genetics. 1989 May;122(1):19-27 - PubMed
    1. Nature. 1989 Jul 20;340(6230):245-6 - PubMed
    1. Mol Cell Biol. 1989 Sep;9(9):3904-10 - PubMed
    1. Mol Cell Biol. 1989 Nov;9(11):5034-44 - PubMed
    1. Cell. 1990 Apr 20;61(2):319-27 - PubMed

Publication types

MeSH terms