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. 1993;28(2):136-9.

[HPLC analysis of alkaloids in Sophora flavescens Ait]

[Article in Chinese]
Affiliations
  • PMID: 8328283

[HPLC analysis of alkaloids in Sophora flavescens Ait]

[Article in Chinese]
L X Jin et al. Yao Xue Xue Bao. 1993.

Abstract

In this paper, a HPLC method was developed for the determination of alkaloids in Sophora flavescens Ait. Experimental evidences indicate that a system of a LiChrosorb-NH2 column (4.0 mm x 250 mm) as stationary phase and CH3CN--H3PO4(pH2)--CH3CH2OH(80:8:10) as mobile phase can separate the five alkaloids, sophocarpine, matrine, sophoridine, oxysophcarpine and oxymatrine very well. This method is very easy and efficient and takes only 15 min for one run.

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