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Comparative Study
. 1993 Jul;175(14):4383-90.
doi: 10.1128/jb.175.14.4383-4390.1993.

Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis

J J Godon  1 , C Delorme, J Bardowski, M C Chopin, S D Ehrlich, P Renault
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Comparative Study

Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis

J J Godon et al. J Bacteriol. 1993 Jul.

Abstract

The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologous to that of the prototroph NCDO2118, which was determined previously. Two nonsense mutations and two small deletions were found in the auxotroph sequence, which might explain the branched-chain amino acid auxotrophy. Nevertheless, the leu genes from the auxotroph appear to be transcribed and regulated similarly to those from the prototroph.

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