Cloning and sequencing of the gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum

Abstract

A 1348 basepair EcoRI fragment of genomic DNA containing the [2Fe-2S] ferredoxin gene from Clostridium pasteurianum has been cloned and sequenced. The translated protein sequence is identical to the sequence of the protein as previously determined by Edman chemistry. Two potential open reading frames occur upstream and downstream of the ferredoxin gene. However, the features surrounding the latter gene strongly suggest that it constitutes a separate transcriptional unit. The sequence data provide no evidence that this ferredoxin is involved in nitrogen fixation.