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. 1977 Jan;25(1):69-72.
doi: 10.1177/25.1.833423.

Immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells

B P Lane, J Elias, E Drummond

Immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells

B P Lane et al. J Histochem Cytochem. 1977 Jan.

Abstract

The ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. Binding to the Z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of sheep anti-rabbit gamma globulin.

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