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. 1993 Jul 15;129(1):153-4.
doi: 10.1016/0378-1119(93)90712-c.

The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and EcoHI

A N Kravetz  1 , M V Zakharova, I V Beletskaya, E V Sineva, M M Denjmuchametov, S I Petrov, L I Glatman, A S Solonin
Affiliations

The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and EcoHI

A N Kravetz et al. Gene. .

Abstract

The restriction endonucleases Eco1831I and EcoHI cleave before the first 5'-cytosine in the recognition sequence 5'-decreases CCSGG--3'/3'--GGSCC increases-5' (where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.

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