Mediation of Trypanosoma cruzi invasion by sialic acid on the host cell and trans-sialidase on the trypanosome
- PMID: 8341323
- DOI: 10.1016/0166-6851(93)90222-j
Mediation of Trypanosoma cruzi invasion by sialic acid on the host cell and trans-sialidase on the trypanosome
Abstract
Trypanosoma cruzi attaches and invades a large variety of mammalian cells. The nature of the cell receptors and of the corresponding parasite counter-receptors that mediate T. cruzi-host cell interaction are not known. Three sialic acid-deficient mutants of Chinese hamster ovary (CHO) cells were used to probe the role of host sialyl residues in T. cruzi infection. All three mutants supported adhesion and infection to a much lower extent than the parental CHO cells. One of the mutants, Lec2, contains sugar chains terminating in non-reducing beta Gal residues, which are acceptors for sialylation by the T. cruzi trans-sialidase. Re-sialylation of Lec2 cells restored T. cruzi adhesion and invasion to about the same extent as wild-type cells. Digestion of wild-type cells with bacterial sialidase reduced T. cruzi interaction but after re-sialylation, the cells were almost as good as control, naturally sialylated parental cells. These results suggest that T. cruzi recognizes sialyl residues on the surface of host cells during invasion. On the other hand, affinity-purified trans-sialidase blocked T. cruzi adherence and invasion of sialylated cells, and had no effect on parasite interaction with sialic acid-deficient Lec2 mutant. Furthermore, 2,3-sialyllactose, a substrate for the trans-sialidase, competitively inhibited T. cruzi invasion of sialylated parental K1 cells, but 2,6-sialyllactose, which does not react with the trans-sialidase, was without effect, as were other sugars that do not contain alpha 2,3 sialyl residues. These results suggest that the trans-sialidase functions as a counter-receptor for trypomastigote binding to alpha 2,3-sialyl receptors on host cells as a prelude to T. cruzi invasion.
Similar articles
-
Mediation of Trypanosoma cruzi invasion by heparan sulfate receptors on host cells and penetrin counter-receptors on the trypanosomes.Mol Biochem Parasitol. 1994 May;65(1):73-83. doi: 10.1016/0166-6851(94)90116-3. Mol Biochem Parasitol. 1994. PMID: 7935630
-
The action of Trypanosoma cruzi trans-sialidase on glycolipids and glycoproteins.Eur J Biochem. 1993 Apr 15;213(2):765-71. doi: 10.1111/j.1432-1033.1993.tb17818.x. Eur J Biochem. 1993. PMID: 8477749
-
A novel cell surface trans-sialidase of Trypanosoma cruzi generates a stage-specific epitope required for invasion of mammalian cells.Cell. 1991 Jun 28;65(7):1117-25. doi: 10.1016/0092-8674(91)90008-m. Cell. 1991. PMID: 1712251
-
Trans-sialidase: a unique enzyme activity discovered in the protozoan Trypanosoma cruzi.FASEB J. 1993 Oct;7(13):1257-64. doi: 10.1096/fasebj.7.13.8405811. FASEB J. 1993. PMID: 8405811 Review.
-
Theft and Reception of Host Cell's Sialic Acid: Dynamics of Trypanosoma Cruzi Trans-sialidases and Mucin-Like Molecules on Chagas' Disease Immunomodulation.Front Immunol. 2019 Feb 6;10:164. doi: 10.3389/fimmu.2019.00164. eCollection 2019. Front Immunol. 2019. PMID: 30787935 Free PMC article. Review.
Cited by
-
Cell signaling during Trypanosoma cruzi invasion.Front Immunol. 2012 Nov 28;3:361. doi: 10.3389/fimmu.2012.00361. eCollection 2012. Front Immunol. 2012. PMID: 23230440 Free PMC article.
-
Ecto-ATPase activity on the surface of Trypanosoma cruzi and its possible role in the parasite-host cell interaction.Parasitol Res. 2003 Oct;91(4):273-82. doi: 10.1007/s00436-003-0965-8. Epub 2003 Aug 22. Parasitol Res. 2003. PMID: 14574556
-
Sialic acids in molecular and cellular interactions.Int Rev Cytol. 1997;175:137-240. doi: 10.1016/s0074-7696(08)62127-0. Int Rev Cytol. 1997. PMID: 9203358 Free PMC article. Review.
-
Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.Parasitol Res. 1995;81(7):560-4. doi: 10.1007/BF00932022. Parasitol Res. 1995. PMID: 7479647
-
Trypanosoma cruzi trans-sialidase: enhancement of virulence in a murine model of Chagas' disease.J Exp Med. 1995 May 1;181(5):1693-703. doi: 10.1084/jem.181.5.1693. J Exp Med. 1995. PMID: 7722448 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources