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. 1993 Aug 16;130(1):141-4.
doi: 10.1016/0378-1119(93)90358-a.

Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2)

G Bucca  1 , C P Smith, M Alberti, G Seidita, R Passantino, A M Puglia
Affiliations

Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2)

G Bucca et al. Gene. .

Abstract

The dnaK homologue of Streptomyces coelicolor A3(2) strain M145 has been cloned and sequenced. Nucleotide sequence analysis of a 2.5-kb region revealed an open reading frame (ORF) encoding a predicted DnaK protein of 618 amino acids (M(r) = 66,274). The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively. The predicted DnaK sequence has a high Lys:Arg ratio which is not typical of streptomycete proteins. The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationally coupled. This organisation differs from that reported for other prokaryotes.

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