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. 1993 Jul 25;21(15):3341-6.
doi: 10.1093/nar/21.15.3341.

A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA

Y Choo  1 , A Klug
Affiliations
Free PMC article

A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA

Y Choo et al. Nucleic Acids Res. .
Free PMC article

Abstract

Zinc fingers of the TFIIIA type are connected by short linker sequences between the structural units. Structural investigations by 2D NMR in solution and by X-ray crystallographic analyses of complexes with DNA point to a passive role for the linkers. We have therefore investigated the influence of the linker sequence on DNA binding using as a model the first three fingers of the protein TFIIIA. Insertion of certain heterologous linkers abolishes binding, and replacement of individual amino acids can reduce binding by factors of up to twenty-four.

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References

    1. J Mol Biol. 1970 Feb 28;48(1):67-83 - PubMed
    1. Science. 1993 Apr 23;260(5107):530-3 - PubMed
    1. Cell. 1984 Jun;37(2):645-52 - PubMed
    1. EMBO J. 1985 Jun;4(6):1609-14 - PubMed
    1. J Mol Biol. 1986 Dec 5;192(3):577-91 - PubMed

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