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. 1993 Aug 1;293 ( Pt 3)(Pt 3):607-11.
doi: 10.1042/bj2930607.

Interactions between active-site-serine beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring: kinetic and molecular modelling studies

A Matagne  1 , J Lamotte-Brasseur, G Dive, J R Knox, J M Frère
Affiliations

Interactions between active-site-serine beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring: kinetic and molecular modelling studies

A Matagne et al. Biochem J. .

Abstract

The interactions between three class A beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring (cefoxitin, moxalactam and temocillin) have been studied. When compared with the situation prevailing with good substrates, both acylation and deacylation steps appeared to be severely impaired. Molecular modelling studies of the structures of the Henri-Michaelis complexes and of the acyl-enzymes indicate a major displacement of the crystallographically observed water molecule which connects the glutamate-166 and serine-70 side chains and underline the role of this water molecule in both reaction steps.

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References

    1. Biochemistry. 1980 Jun 24;19(13):2895-901 - PubMed
    1. Nature. 1992 Oct 22;359(6397):700-5 - PubMed
    1. Antimicrob Agents Chemother. 1982 Sep;22(3):453-60 - PubMed
    1. Eur J Biochem. 1984 Oct 15;144(2):333-8 - PubMed
    1. Crit Rev Microbiol. 1985;11(4):299-396 - PubMed

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