Intra-peritoneal free elastase in CAPD peritonitis

Abstract

Neutrophil (PMN) recruitment into the peritoneum during acute bacterial peritonitis is an important part of the host defense barrier in CAPD patients. However, the subsequent phagocytosis of bacteria may also lead to PMN degranulation and the release of lysosomal enzymes. We determined the concentration of neutrophil elastase, both in complex with its natural inhibitor alpha 1Pi (E alpha 1Pi), and in uncomplexed, free form, in infected and normal CAPD peritoneal fluid by ELISA. In addition elastase activity was estimated in a casein degradation assay. Infected fluid contained a median (range) of 1.4 nM (0 to 9.2) free elastase by ELISA and 1.2 nM (0 to 11.9) activity. There were strong correlations between the peritoneal leukocyte count and both immunoreactive elastase and activity (r = 0.816, P < 0.001, 0.687, P < 0.01, respectively). In contrast, normal fluid contained 0.0 nM (0 to 0.32) immunoreactive elastase (P < 0.01) and 0.0 nM (0 to 0.6) elastase activity (P < 0.001). E alpha 1Pi complexes were raised significantly during peritonitis at 6.2 nM (0 to 34.3) and were barely detectable in normal fluid 0.0 nM (0 to 0.17; P < 0.005). The study shows that small but significant quantities of uninhibited elastase can be detected in the peritoneal fluid of CAPD patients with acute bacterial peritonitis. This observation may have important implications for the pathogenesis of peritoneal membrane damage and the phlogistic response to infection.