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. 1993 May;29(3):233-5.
doi: 10.1006/plas.1993.1025.

Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants

T L Bannam  1 , J I Rood
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Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants

T L Bannam et al. Plasmid. 1993 May.

Abstract

Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens.

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