doi: 10.1128/aem.59.7.2339-2342.1993.
Colony immunoblot assay of botulinal toxin
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- PMID: 8357267
- PMCID: PMC182282
- DOI: 10.1128/aem.59.7.2339-2342.1993
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Colony immunoblot assay of botulinal toxin
Appl Environ Microbiol.
1993 Jul.
Abstract
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.
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