Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jul;37(7):1400-5.
doi: 10.1128/AAC.37.7.1400.

Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923

G E Dale  1 , R L Then, D Stüber
Affiliations

Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923

G E Dale et al. Antimicrob Agents Chemother. 1993 Jul.

Abstract

The gene for the trimethoprim-sensitive (Tmps) chromosomal dihydrofolate reductase (DHFR) of Staphylococcus aureus ATCC 25923 was cloned and characterized. The structural gene encodes a polypeptide of 159 amino acid residues and has a calculated molecular weight of 18,251. The amino acid sequences of this Tmps DHFR and those of the trimethoprim-resistant type S1 DHFR encoded by transposon Tn4003 are 80% identical. In contrast to the trimethoprim-resistant enzyme, the Tmps DHFR can be highly overexpressed in Escherichia coli, with most of the recombinant protein occurring in a soluble and an active form.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 - PubMed
    1. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 - PubMed
    1. EMBO J. 1984 Dec 20;3(13):3143-8 - PubMed
    1. J Antimicrob Chemother. 1986 Sep;18(3):421-4 - PubMed
    1. Microbiol Rev. 1987 Mar;51(1):88-134 - PubMed

MeSH terms

Substances