Non-distorted assemblage of the digital images of adjacent fields in histological sections

Abstract

In the following article a method is described for the distortion-free reassemblage of adjacent digitised microscopic images of histological sections. The correct positioning of the microscopic specimen is achieved via a computer-controlled scanning stage. During an internal calibration step the correction function for the image distortions and the parameters of the transformation scanning-stage-to image-coordinate system are determined. In the subsequent application phase the new positions the scanning stage has to move to can now be computed in such a way that the distortion-corrected digitised images of the section parts under consideration neatly fit together to one composite image.