Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jul;2(4):183-90.
doi: 10.1007/BF01977348.

Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells

J W McCarrick 3rd  1 , J R Parnes, R H Seong, D Solter, B B Knowles
Affiliations

Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells

J W McCarrick 3rd et al. Transgenic Res. 1993 Jul.

Abstract

The diphtheria toxin A-chain gene was used in a positive-negative selection gene targeting vector to alter the CD4 gene which is transcriptionally silent in mouse embryonic stem cells. Expression of the toxin gene was driven by a constitutively active enhancer, yet the targeting construct exhibited only minimal transient toxicity while enriching for targeted clones 9- to 29-fold. Germline transmission of the stem cell-derived genome was obtained. These data suggest the usefulness of this diphtheria toxin A-chain cassette in replacement-type positive-negative selection vectors. Its potential for novel applications, particularly in the enrichment for 'hit-and-run' insertion-type vectors, is discussed.

PubMed Disclaimer

References

    1. Annu Rev Biochem. 1986;55:195-224 - PubMed
    1. Dev Biol. 1991 Dec;148(2):508-16 - PubMed
    1. Nature. 1986 Oct 2-8;323(6087):445-8 - PubMed
    1. Cell. 1992 Apr 17;69(2):251-64 - PubMed
    1. Cell. 1987 Jul 31;50(3):435-43 - PubMed

Publication types

LinkOut - more resources