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. 1993 Sep 13;330(2):197-200.
doi: 10.1016/0014-5793(93)80272-v.

Interaction of GTP-binding protein Gq with photoactivated rhodopsin in the photoreceptor membranes of crayfish

A Terakita  1 , T Hariyama, Y Tsukahara, Y Katsukura, H Tashiro
Affiliations
Free article

Interaction of GTP-binding protein Gq with photoactivated rhodopsin in the photoreceptor membranes of crayfish

A Terakita et al. FEBS Lett. .
Free article

Abstract

Interaction of G-protein with photoactivated rhodopsin (Rh*) in crayfish photoreceptor membranes was investigated by immunoprecipitation using an antibody against rhodopsin. Two kinds of protein were co-precipitated with rhodopsin. One is an alpha subunit of class-q G-protein (42 kDa, CGq alpha) which showed light-induced, dose-dependent binding to rhodopsin, and the other is an actin-like protein (44 kDa) with light-independent binding. Most of the CGq alpha was available for binding to Rh* but was dissociated from Rh* in the presence of GTP gamma S. These findings demonstrate that, in the crayfish photoreceptor, a Gq class of G-protein is activated by Rh*.

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