Apoptosis is induced by beta-amyloid in cultured central nervous system neurons
- PMID: 8367446
- PMCID: PMC47265
- DOI: 10.1073/pnas.90.17.7951
Apoptosis is induced by beta-amyloid in cultured central nervous system neurons
Abstract
The molecular mechanism responsible for the neurodegeneration in Alzheimer disease is not known; however, accumulating evidence suggests that beta-amyloid peptide (A beta P) contributes to this degeneration. We now report that synthetic A beta Ps trigger the degeneration of cultured neurons through activation of an apoptotic pathway. Neurons treated with A beta Ps exhibit morphological and biochemical characteristics of apoptosis, including membrane blebbing, compaction of nuclear chromatin, and internucleosomal DNA fragmentation. Aurintricarboxylic acid, an inhibitor of nucleases, prevents DNA fragmentation and delays cell death. Our in vitro results suggest that apoptosis may play a role in the neuronal loss associated with Alzheimer disease.
Similar articles
-
Beta-amyloid peptide fragment 31-35 induces apoptosis in cultured cortical neurons.Neuroscience. 1999;92(1):177-84. doi: 10.1016/s0306-4522(98)00727-1. Neuroscience. 1999. PMID: 10392840
-
Ultrastructural analysis of beta-amyloid-induced apoptosis in cultured hippocampal neurons.Brain Res. 1994 Oct 24;661(1-2):147-56. doi: 10.1016/0006-8993(94)91191-6. Brain Res. 1994. PMID: 7834365
-
The nonfibrillar amyloid beta-peptide induces apoptotic neuronal cell death: involvement of its C-terminal fusogenic domain.J Neurochem. 1999 Oct;73(4):1626-34. doi: 10.1046/j.1471-4159.1999.0731626.x. J Neurochem. 1999. PMID: 10501209
-
A potential role for apoptosis in neurodegeneration and Alzheimer's disease.Mol Neurobiol. 1995 Feb;10(1):19-45. doi: 10.1007/BF02740836. Mol Neurobiol. 1995. PMID: 7598831 Review.
-
The beta-amyloid peptide, peptide self-assembly, and the emergence of biological activities. A new principle in peptide function and the induction of neuropathology.Ann N Y Acad Sci. 1997 Apr 24;814:1-16. doi: 10.1111/j.1749-6632.1997.tb46140.x. Ann N Y Acad Sci. 1997. PMID: 9160955 Review. No abstract available.
Cited by
-
Beta-amyloid-induced synthesis of the ganglioside GD3 is a requisite for cell cycle reactivation and apoptosis in neurons.J Neurosci. 2002 May 15;22(10):3963-8. doi: 10.1523/JNEUROSCI.22-10-03963.2002. J Neurosci. 2002. PMID: 12019315 Free PMC article.
-
Reduction of trophic support enhances apoptosis in PC12 cells expressing Alzheimer's APP mutation and sensitizes cells to staurosporine-induced cell death.J Mol Neurosci. 2002 Jun;18(3):189-201. doi: 10.1385/JMN:18:3:189. J Mol Neurosci. 2002. PMID: 12059037
-
Caspase-mediated cleavage of glial fibrillary acidic protein within degenerating astrocytes of the Alzheimer's disease brain.Am J Pathol. 2006 Mar;168(3):936-46. doi: 10.2353/ajpath.2006.050798. Am J Pathol. 2006. PMID: 16507909 Free PMC article.
-
Monascus pigment rubropunctatin derivative FZU-H reduces Aβ(1-42)-induced neurotoxicity in Neuro-2A cells.RSC Adv. 2018 May 14;8(31):17389-17398. doi: 10.1039/c8ra02365d. eCollection 2018 May 9. RSC Adv. 2018. PMID: 35539257 Free PMC article.
-
Adventures in neural plasticity, aging, and neurodegenerative disorders aboard the CWC beagle.Neurochem Res. 2003 Nov;28(11):1631-7. doi: 10.1023/a:1026000703290. Neurochem Res. 2003. PMID: 14584817
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources