Pulsed-field gel electrophoretic analysis of Campylobacter jejuni DNA for use in epidemiological studies
- PMID: 8370943
- DOI: 10.1016/0163-4453(93)93628-h
Pulsed-field gel electrophoretic analysis of Campylobacter jejuni DNA for use in epidemiological studies
Abstract
The cleavage patterns of the genomic DNA of 42 clinical isolates of Campylobacter jejuni determined by pulsed-field gel electrophoresis (PFGE) were compared with their Lior and TCK serotypes. The fragment patterns of DNA obtained with SalI and SmaI restriction enzymes did not always accord with the corresponding serotypes but strains of the same serotype could be further divided into subtypes by their cleavage patterns. This PFGE method may prove useful for subclassifying C. jejuni.
Similar articles
-
Discrimination by means of pulsed-field gel electrophoresis between strains of Campylobacter jejuni Lior type 4 derived from sporadic cases and from outbreaks of infection.J Infect. 1994 Sep;29(2):183-7. doi: 10.1016/s0163-4453(94)90722-6. J Infect. 1994. PMID: 7806881
-
Macro-fingerprinting analysis at the chromosomal genomic DNA level of isolates of thermophilic Campylobacter coli and C. jejuni, by pulsed-field gel electrophoresis.Cytobios. 1994;78(313):115-22. Cytobios. 1994. PMID: 8070288
-
Lineages within Campylobacter jejuni defined by numerical analysis of pulsed-field gel electrophoretic DNA profiles.J Med Microbiol. 1997 Feb;46(2):157-63. doi: 10.1099/00222615-46-2-157. J Med Microbiol. 1997. PMID: 9060876
-
Comparison of a commercial test for serotyping heat-stable antigens of Campylobacter jejuni with genotyping by pulsed-field gel electrophoresis.J Med Microbiol. 1999 Jul;48(7):617-621. doi: 10.1099/00222615-48-7-617. J Med Microbiol. 1999. PMID: 10403411
-
Microscopic behaviour of DNA during electrophoresis: electrophoretic orientation.Q Rev Biophys. 1991 May;24(2):103-64. doi: 10.1017/s0033583500003395. Q Rev Biophys. 1991. PMID: 1924681 Review. No abstract available.
Cited by
-
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.J Clin Microbiol. 1995 Sep;33(9):2233-9. doi: 10.1128/jcm.33.9.2233-2239.1995. J Clin Microbiol. 1995. PMID: 7494007 Free PMC article. Review. No abstract available.
-
Evidence of genomic instability in Campylobacter jejuni isolated from poultry.Appl Environ Microbiol. 1998 May;64(5):1816-21. doi: 10.1128/AEM.64.5.1816-1821.1998. Appl Environ Microbiol. 1998. PMID: 9572956 Free PMC article.
-
Genotyping of Campylobacter spp.Appl Environ Microbiol. 2000 Jan;66(1):1-9. doi: 10.1128/AEM.66.1.1-9.2000. Appl Environ Microbiol. 2000. PMID: 10618195 Free PMC article. Review. No abstract available.
-
Comparative fingerprinting analysis of Campylobacter jejuni subsp. jejuni strains by amplified-fragment length polymorphism genotyping.J Clin Microbiol. 2000 Sep;38(9):3379-87. doi: 10.1128/JCM.38.9.3379-3387.2000. J Clin Microbiol. 2000. PMID: 10970387 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources