Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1993 Sep 10;74(5):919-28.
doi: 10.1016/0092-8674(93)90471-2.

An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling

G M Thomas  1 , E Cunningham, A Fensome, A Ball, N F Totty, O Truong, J J Hsuan, S Cockcroft
Affiliations
Comparative Study

An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling

G M Thomas et al. Cell. .

Abstract

Transmembrane signaling by the phospholipase C-beta (PLC-beta) pathway is known to require at least three components: the receptor, the G protein, and the PLC. Recent studies have indicated that if the cytosol is allowed to leak out of HL60 cells, then G protein-stimulated PLC activity is greatly diminished, indicating an essential role for a cytosolic component(s). We now report the complete purification of one component based on its ability to reconstitute GTP gamma S-mediated PLC activity and identify it as the phosphatidylinositol transfer protein (PI-TP). Based on the in vitro effects of PI-TP, we surmise that it is involved in transporting PI from intracellular compartments for conversion to PI bisphosphate (PIP2) prior to hydrolysis by PLC-beta 2/PLC-beta 3, the endogenous PLC isoforms present in these cells.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources