doi: 10.1016/0378-4347(93)80402-p.
Liquid chromatography with electrochemical detection for quantitation of bound choline liberated by phospholipase D hydrolysis from phospholipids containing choline in rat plasma
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- PMID: 8376514
- DOI: 10.1016/0378-4347(93)80402-p
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Liquid chromatography with electrochemical detection for quantitation of bound choline liberated by phospholipase D hydrolysis from phospholipids containing choline in rat plasma
J Chromatogr.
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Abstract
This report describes the optimal conditions for the determination of bound choline in rat plasma. The method used was based on the liberation of choline by phospholipase D from phospholipids containing choline in plasma, followed by high-performance liquid chromatographic analysis. Normal concentrations of total, free and bound choline in rat plasma were found to be 1278.7 +/- 132.5, 11.5 +/- 2.2 and 1267.2 +/- 125.5 nmol/ml, respectively. The described procedure has the advantages of rapidity, specificity, excellent precision and the need for only a small amount of the sample.
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