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. 1993 Sep;92(3):1580-6.
doi: 10.1172/JCI116739.

Efficient adenoviral-mediated ornithine transcarbamylase expression in deficient mouse and human hepatocytes

M A Morsy  1 , E L Alford, A Bett, F L Graham, C T Caskey
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Efficient adenoviral-mediated ornithine transcarbamylase expression in deficient mouse and human hepatocytes

M A Morsy et al. J Clin Invest. 1993 Sep.

Abstract

100% of primary human hepatocytes infected with an adenoviral vector carrying beta-galactosidase expressed the exogenous gene. Expression was also achieved in > 40% of adult mouse hepatocytes in vivo. Normal levels of activity were achieved in mouse ornithine transcarbamylase (OTC)-deficient primary hepatocytes using another adenoviral vector carrying human OTC cDNA. Study of OTC-deficient primary human hepatocytes from a single patient confirmed the utility of adenoviral delivery of OTC. We describe adenoviral-mediated exogenous gene expression in human and mouse hepatocytes in vitro and in mouse liver in vivo. Data suggest that adenoviral vectors may be useful for correcting OTC deficiency.

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