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. 1993;128(1-2):65-79.
doi: 10.1007/BF01309789.

Proteins specifically binding to the 3' untranslated region of hepatitis A virus RNA in persistently infected cells

J P Nüesch  1 , M Weitz, G Siegl
Affiliations

Proteins specifically binding to the 3' untranslated region of hepatitis A virus RNA in persistently infected cells

J P Nüesch et al. Arch Virol. 1993.

Abstract

Establishment of persistency is the common result of hepatitis A virus (HAV) infection in most HAV/cell culture systems. Previous studies provided evidence that shortly before or concomitantly with establishment of persistent infections synthesis of viral RNA is down-regulated. This may be an effect of regulating factors. Using RNA/protein binding assays it was shown that, at the critical time during virus replication, proteins accumulate which interact specifically with a distinct nucleotide sequence (HPE) within the 3' non-coding region of the HAV genome and/or (HME) within the 5' terminal region of the HAV antigenome. The sequences consist of 23 nucleotides (HPE: 5'-AAAUUUUCUUAAAAUUUCUGAGG-3'; HME: 5'-CCUCAGAAAUUUUAAGAAAAUUU-3'). A sequence with 79% similarity was found in the corresponding 3' non-coding region of poliovirus type I (Sabin) RNA. The latter sequence was shown to bind proteins from HAV infected cells but comparable proteins were absent in cells infected poliovirus.

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References

    1. J Mol Biol. 1984 Apr 25;174(4):561-85 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Virol Methods. 1984 Aug;9(1):53-67 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7590-4 - PubMed
    1. Biotechniques. 1988 Jun;6(6):544-6, 549 - PubMed

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