doi: 10.1006/bbrc.1993.1003.
HepG2 cells: an in vitro model for P450-dependent metabolism of acetaminophen
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- PMID: 8380689
- DOI: 10.1006/bbrc.1993.1003
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HepG2 cells: an in vitro model for P450-dependent metabolism of acetaminophen
Biochem Biophys Res Commun.
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Abstract
The human hepatoma cell line, HepG2, retains many cellular functions often lost by cells in culture. This research examined the constitutive bioactivation of acetaminophen and P450-dependent activity in microsomes from HepG2 cells and the effect of 0.1% acetone pretreatment on these activities. Low levels of acetaminophen bioactivation, P450 IIE1 activity, and P450 IA1-IA2 activity were demonstrated in non-induced HepG2 microsomes. Acetone increased acetaminophen bioactivation and IIE1-dependent metabolism but not P450 IA1-IA2-dependent activity. Thus, HepG2 cells may provide an in vitro model for assessing human xenobiotic metabolism of acetaminophen and other drugs.
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