Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jan 15;190(1):15-9.
doi: 10.1006/bbrc.1993.1003.

HepG2 cells: an in vitro model for P450-dependent metabolism of acetaminophen

A L Roe  1 , J E Snawder, R W Benson, D W Roberts, D A Casciano
Affiliations

HepG2 cells: an in vitro model for P450-dependent metabolism of acetaminophen

A L Roe et al. Biochem Biophys Res Commun. .

Abstract

The human hepatoma cell line, HepG2, retains many cellular functions often lost by cells in culture. This research examined the constitutive bioactivation of acetaminophen and P450-dependent activity in microsomes from HepG2 cells and the effect of 0.1% acetone pretreatment on these activities. Low levels of acetaminophen bioactivation, P450 IIE1 activity, and P450 IA1-IA2 activity were demonstrated in non-induced HepG2 microsomes. Acetone increased acetaminophen bioactivation and IIE1-dependent metabolism but not P450 IA1-IA2-dependent activity. Thus, HepG2 cells may provide an in vitro model for assessing human xenobiotic metabolism of acetaminophen and other drugs.

PubMed Disclaimer

LinkOut - more resources