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. 1993 Jan;37(1):126-7.
doi: 10.1128/AAC.37.1.126.

A single point mutation in the DNA gyrase A protein greatly reduces binding of fluoroquinolones to the gyrase-DNA complex

C J Willmott  1 , A Maxwell
Affiliations

A single point mutation in the DNA gyrase A protein greatly reduces binding of fluoroquinolones to the gyrase-DNA complex

C J Willmott et al. Antimicrob Agents Chemother. 1993 Jan.

Abstract

Binding of the quinolone drug norfloxacin to gyrase and DNA has been investigated. We have detected binding to gyrase-DNA complex but find no significant binding to either gyrase or DNA alone. Enzyme containing gyrase A protein with the mutation Ser-83 to Trp (conferring quinolone resistance) showed greatly reduced drug binding.

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References

    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4165-9 - PubMed
    1. J Biol Chem. 1984 Dec 10;259(23):14472-80 - PubMed
    1. Mol Gen Genet. 1988 Jan;211(1):1-7 - PubMed
    1. J Biol Chem. 1989 Feb 15;264(5):2973-8 - PubMed

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