Structural analysis and expression of a chromosomal gene encoding an avian Na+/K(+)-ATPase beta 1-subunit

Abstract

Chicken chromosomal DNA encoding the Na+/K(+)-ATPase beta 1-subunit was cloned and characterized. Its exon-intron structure is identical to mammalian (human and rat) beta 1-subunit genes. The transcription initiation site, TATA box, and an ATTGG (antisense CCAAT) sequence follow approximately 1 kilobase of GC-rich 5' upstream sequence that contains many consensus sequences for transcription factors whose relative positions are conserved between human and chicken genes. When this beta 1-subunit gene was stably incorporated into mouse L cells and C2C12 cells, the avian beta 1-subunit was expressed under the control of the its own promoter.