Detection of PML/retinoic acid receptor a gene rearrangements by polymerase chain reaction using genomic DNA in patients with acute promyelocytic leukemia
- PMID: 8385082
- PMCID: PMC5919129
- DOI: 10.1111/j.1349-7006.1993.tb02841.x
Detection of PML/retinoic acid receptor a gene rearrangements by polymerase chain reaction using genomic DNA in patients with acute promyelocytic leukemia
Abstract
Breakpoints of the 15;17 translocation in patients with acute promyelocytic leukemia (APL) have been identified within PML and retinoic acid receptor a (RARA) genes in chromosomes 15 and 17, respectively. A wide heterogeneity was observed in the breakpoints on the PML and RARA genes. Therefore, amplification of the breakpoints region by polymerase chain reaction (PCR) with genomic DNA has been considered to be difficult. In the present study, a method was developed to detect the 15;17 translocation with genomic DNA. Of 13 patients with APL, four were detected to have the rearrangement of genomic DNA. At present, reverse transcriptase-polymerase chain reaction analysis is one of the methods available for diagnosis and detection of the residual leukemic cells in APL. In this study, PCR analysis using genomic DNA of APL cells is proved to be useful for identifying the breakpoints of the PML and the RARA genes. Furthermore, this method is applicable to patients for whom RNA samples of the leukemic cells are not available.
References
-
- ) Rowley , J. D. , Golomb , H. M. and Dougherty , C.15/17 translocation, a consistent chromosomal change in acute promyelocytic leukemia . Lancet , i , 549 – 550 ( 1977. ). - PubMed
-
- ) Bennett , J. M. , Catovsky , D. , Daniel , M. T. , Flandrin , G. , Galton , D. A. G. , Gralnick , H. R. and Sultan , C.Proposals for the classification of the acute leukemias . Br. J. Haematol. , 33 , 451 – 458 ( 1976. ). - PubMed
-
- ) The Fourth International Workshop on Chromosomes in Leukemia. Chromosomes in acute promyelocytic leukemia . Cancer Genet. Cytogenet. , 11 , 288 – 293 ( 1984. ). - PubMed
-
- ) Borrow , H. , Goddard , A. D. , Sheer , D. and Solomon , E.Molecular analysis of acute promyelocytic leukemia breakpoint cluster region on chromosome 17 . Science , 249 , 1577 – 1580 ( 1990. ). - PubMed
-
- ) de The , H. , Chomienne , C. , Lanotte , M. , Degos , L. and Dejean , A.The t(15;17) translocation of acute promyelocytic leukaemia fuses the retinoic acid receptor α gene to a novel transcribed locus . Nature , 347 , 558 – 561 ( 1990. ). - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources