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Comparative Study
. 1993 Apr;31(4):986-9.
doi: 10.1128/jcm.31.4.986-989.1993.

Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus

J F Ridpath  1 , S R Bolin, J Katz
Affiliations
Comparative Study

Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus

J F Ridpath et al. J Clin Microbiol. 1993 Apr.

Abstract

Primers and probes derived from conserved sequences located in the 5' noncoding region of pestiviruses were evaluated for detection of bovine viral diarrhea virus. With these reagents, hybridization and polymerase chain reaction tests detected 62 of 90 and 90 of 90 bovine viral diarrhea virus isolates, respectively. A quick lysis method for preparing RNA for use in polymerase chain reaction amplification also was evaluated.

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