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Comparative Study
. 1993 Feb 15;209(1):6-8.
doi: 10.1006/abio.1993.1075.

Assay of nucleoside diphosphate kinase in microtiter plates using a peroxidase-coupled method

I Lascu  1 , K LeBlay, M L Lacombe, E Presecan, M Véron
Affiliations
Comparative Study

Assay of nucleoside diphosphate kinase in microtiter plates using a peroxidase-coupled method

I Lascu et al. Anal Biochem. .

Abstract

A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for testing a large number of samples generated by chromatographic techniques or for screening purposes.

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