Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Mar;18(3):271-5.
doi: 10.1007/BF00969082.

Cyclic AMP regulation of the human choline acetyltransferase gene

Y P Li  1 , E E Baetge, L B Hersh
Affiliations

Cyclic AMP regulation of the human choline acetyltransferase gene

Y P Li et al. Neurochem Res. 1993 Mar.

Abstract

Regulation of the human choline acetyltransferase gene by 8-bromo-cAMP was investigated by transfecting into NS-20Y cells choline acetyltransferase promoter sequences fused to the reporter gene firefly luciferase. Promoter activity was localized to nucleotides -163 to +73. This region of the gene responded to 8-bromo-cAMP in a similar fashion as the endogenous enzyme. However, nearly the same induction was observed for constructs containing unrelated promoters. Furthermore, fusion of the choline acetyltransferase gene to the thymidine kinase promoter yielded no increased induction by 8-bromo-cAMP. These results suggest that cAMP regulates choline acetyltransferase gene transcription through an indirect mechanism.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Cytobios. 1973 Sep-Oct;8(29):75-80 - PubMed
    1. J Biol Chem. 1988 Dec 5;263(34):18574-7 - PubMed
    1. Science. 1983 Mar 11;219(4589):1184-90 - PubMed
    1. Brain Res. 1983 Jul;285(1):45-52 - PubMed
    1. Biotechniques. 1988 May;6(5):454-8 - PubMed

Publication types

LinkOut - more resources