Induction of D-aldohexoside:cytochrome c oxidoreductase in Agrobacterium tumefaciens

Abstract

D-Aldohexopyranoside:cytochrome c oxidoreductase (ACO) was strongly induced by cellobiose, alpha-methylglucoside, beta-methylglucoside, kojibiose, and sophorose. Induction was rapid, and ACO was readily detectable within 10 min after addition of cellobiose as inducer. Although not measurable for 30 to 40 min after addition of inducer, once started, the rate of induction with alpha-methylglucoside equaled or even exceeded that obtained with cellobiose. Induction by sucrose, maltose, alpha-alpha-trehalose, melibiose, and lactose was weak. In general, the active ACO inducers were poor glycosidase inducers; the converse also appeared to be true. Although ACO induction was not repressed by D-glucose, it was repressed by succinate, malate, and fumarate.