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. 1993 Apr;9(2):123-5.
doi: 10.1093/bioinformatics/9.2.123.

Design of primers for PCR amplification of highly variable genomes

J Dopazo  1 , A Rodríguez, J C Sáiz, F Sobrino
Affiliations

Design of primers for PCR amplification of highly variable genomes

J Dopazo et al. Comput Appl Biosci. 1993 Apr.

Abstract

A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.

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