Thyroxine 5'-deiodinase type II activity in chick pineal and Harderian gland: nyctohemeral rhythmicity and its regulation by noradrenergic input

Abstract

Circadian rhythmicity of type II thyroxine 5'-deiodinase (5'-D) activity was studied in the pineal gland and Harderian glands of chicks. Only Harderian 5'-D activity showed a nyctohemeral rhythmicity with a maximal peak during the day time (1300), while no rhythm of enzyme activity was found in the pineal gland. Besides type II 5'-D activity, we found high basal levels of the type I isoenzyme in both glands; this activity was specifically suppressed by the addition of 6-n-propyl-thiouracil (PTU). However, day-night differences in Harderian 5'-D activity were maintained even after the addition of PTU. This activity was not affected for either continuous light exposure or darkness during the day. 5'-D activity seems to be regulated by the noradrenergic input, since the enzymatic activity was stimulated by a beta-adrenergic agonist, isoproterenol, and by the alpha-adrenergic agonist, phenylephrine, in both pineal and Harderian glands. Both drugs affected 5'-D activity in the Harderian gland by stimulating the enzyme activity over basal levels.