Identification of novel members of G-protein coupled receptor superfamily expressed in bovine taste tissue

Abstract

Using reverse transcription-polymerase chain reaction (RT-PCR) and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Physiological functions of these receptors in taste cells are discussed.