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. 1993 Jul 15;194(1):57-64.
doi: 10.1006/bbrc.1993.1784.

Spin trapping of superoxide released by opsonized asbestos from human promyelocytic leukemia cell line, HL60

T Takeuchi  1 , K Morimoto, H Kosaka, T Shiga
Affiliations

Spin trapping of superoxide released by opsonized asbestos from human promyelocytic leukemia cell line, HL60

T Takeuchi et al. Biochem Biophys Res Commun. .

Abstract

By ESR using 5,5-dimethyl-1-pyrroline-1-oxide as a spin trap, superoxide (O2-) production was proved upon stimulation of dimethyl sulfoxide-differentiated HL60 by crocidolite opsonized with fresh or refrigerated serum, as well as by phorbol myristate acetate (PMA). Crocidolite, unopsonized or opsonized with frozen-thawed or heat-inactivated serum, did not induce O2- release. Addition of iron chelators or superoxide dismutase inhibited O2- release completely. Neither undifferentiated nor PMA-differentiated HL60 released O2- upon stimulation with opsonized crocidolite.

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