Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jul;22(4):625-33.
doi: 10.1007/BF00047403.

Behaviour of modified Ac elements in flax callus and regenerated plants

E J Finnegan  1 , G J Lawrence, E S Dennis, J G Ellis
Affiliations

Behaviour of modified Ac elements in flax callus and regenerated plants

E J Finnegan et al. Plant Mol Biol. 1993 Jul.

Abstract

The Ac element of maize has been modified by deletion of 537 bases (delta NaeAc) from the untranslated leader of the transposase gene. In a second modification the cauliflower mosaic virus 35S promoter has been inserted into the truncated leader of delta NaeAc, 21 bases upstream of the natural translation start. The activity of these modified elements has been compared with that of the unmodified element in transgenic flax. Deletion of sequences in the untranslated leader only marginally increased transposition in callus while insertion of the 35S promoter enhanced transposition frequency 7-8-fold. Increased transposition correlated with increased transcription of the transposase gene. The presence of a 35S promoter upstream of the transposase gene, but outside the Ac element, also enhanced both transcription and transposition.

PubMed Disclaimer

References

    1. EMBO J. 1987 Jun;6(6):1555-63 - PubMed
    1. Genetics. 1987 Sep;117(1):109-16 - PubMed
    1. Plant Cell Rep. 1990 Nov;9(7):406-9 - PubMed
    1. Nucleic Acids Res. 1991 Feb 11;19(3):617-22 - PubMed
    1. Plant Cell. 1989 Aug;1(8):757-64 - PubMed

LinkOut - more resources