Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Aug 16;130(1):151-2.
doi: 10.1016/0378-1119(93)90360-f.

M13 and pUC vectors with new unique restriction sites for cloning

V Benes  1 , Z Hostomský, L Arnold, V Paces
Affiliations

M13 and pUC vectors with new unique restriction sites for cloning

V Benes et al. Gene. .

Abstract

Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessential part of the vector molecule was changed into a BssHII site, so that the NarI site in the MCS became unique.

PubMed Disclaimer

Substances

LinkOut - more resources