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. 1993 Jun;43(1):77-84.
doi: 10.1016/0166-0934(93)90091-5.

A new method of RNA preparation for detection of hepatitis A virus in environmental samples by the polymerase chain reaction

F Yang  1 , X Xu
Affiliations

A new method of RNA preparation for detection of hepatitis A virus in environmental samples by the polymerase chain reaction

F Yang et al. J Virol Methods. 1993 Jun.

Abstract

A new method based on RNA preparation from shellfish by polyethylene glycol (PEG) precipitation and trichloroacetic acid (TCA) extraction was developed for the detection of hepatitis A virus (HAV) by the polymerase chain reaction (PCR). The extraction provides high yield and the extracted RNA is undegraded. This method proved to be particularly useful for detection of RNA viruses from environmental samples by PCR.

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