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. 1993 Jun;16(6):565-7.
doi: 10.1248/bpb.16.565.

Inhibition of SV40 DNA replication in vitro by chlorpromazine

H Hirai  1 , S Takeda, S Natori, K Sekimizu
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Free article

Inhibition of SV40 DNA replication in vitro by chlorpromazine

H Hirai et al. Biol Pharm Bull. 1993 Jun.
Free article

Abstract

We examined the inhibitory effect of chlorpromazine, a phenothiazine derivative, on SV40 DNA replication in a HeLa cell extract. The inhibition by chlorpromazine was reversed by cardiolipin, phosphatidylinositol, phosphatidylglycerol, and phosphatidylserine. Studies on a staged reaction showed that the pre-elongation step was sensitive to chlorpromazine: chlorpromazine-resistant DNA synthesis was observed when the drug was added after the pre-elongation step. Analysis of replication products by agarose gel electrophoresis revealed that some steps of DNA chain elongation and maturation of closed circular forms were also sensitive to this drug.

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