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. 1993 Aug;15(2):255-9.

Epstein-Barr virus-derived vectors for transient and stable expression of recombinant proteins

G Cachianes  1 , C Ho, R F Weber, S R Williams, D V Goeddel, D W Leung
Affiliations
  • PMID: 8396947

Epstein-Barr virus-derived vectors for transient and stable expression of recombinant proteins

G Cachianes et al. Biotechniques. 1993 Aug.

Abstract

The transient expression level of foreign proteins in primate cells can be enhanced by incorporating the replication elements derived from Epstein-Barr virus (EBV). Specifically, we have constructed expression plasmids with the replication origin region (OriP) from EBV and an adenovirus-transformed cell line that expresses the EBV nuclear antigens-1 (EBNA-1). As EBV vectors can replicate as episomes in the nuclei, such vectors can have a stable transfection efficiency as high as 25% and provide a straightforward way of obtaining large amounts of recombinant proteins transiently or stably.

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