[New site site-specific endonuclease and methylase from Bacillus licheniformis 736]
[Article in
Russian]
- PMID: 8399763
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[New site site-specific endonuclease and methylase from Bacillus licheniformis 736]
[Article in
Russian]
Biokhimiia.
1993 Aug.
Abstract
The site-specific endonuclease R Bli736I and methylase M Bli736I have been isolated from the Bacillus licheniformis strain 736 by blue-agarose, hydroxyapatite-Ultragel and heparin-Sepharose chromatography. The enzymes are free from interfering impurities. R Bli736I recognizes the 5'-GGTCTCN-3' decreases and decreases 5'-NNNNNGAGACC-3' sequences on the DNA and cleaves the DNA as indicated by arrows to form single-stranded 4-nucleotide 5'-protruding termini. This enzyme is an isoschizomer of Eco3II isolated from E. coli.
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