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. 1993 Jul;3(4):487-93.
doi: 10.1007/BF00176014.

A simple and sensitive experiment for measurement of JCC couplings between backbone carbonyl and methyl carbons in isotopically enriched proteins

S Grzesiek  1 , G W Vuister, A Bax
Affiliations

A simple and sensitive experiment for measurement of JCC couplings between backbone carbonyl and methyl carbons in isotopically enriched proteins

S Grzesiek et al. J Biomol NMR. 1993 Jul.

Abstract

A simple 2D difference experiment is described that allows quantitative measurement of 13C-13C J couplings between backbone carbonyl and side-chain carbons. Precise 3JCC values were measured from data recorded in just 2 h for a 1-mM solution of the 20-kD complex between the protein calmodulin and a 26-residue synthetic peptide. The J couplings aid in determining the chi 1 angles of valine, isoleucine and threonine residues, and in making stereospecific assignments of the Val C gamma methyl groups. Error analysis indicates that the uncertainty in the derived J couplings is generally less than ca. 0.3 Hz.

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References

    1. J Biomol NMR. 1992 Sep;2(5):527-33 - PubMed
    1. Science. 1992 Aug 28;257(5074):1251-5 - PubMed
    1. Science. 1992 May 1;256(5057):632-8 - PubMed
    1. Biochemistry. 1985 Dec 31;24(27):8152-7 - PubMed

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