Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Sep;5(9):1081-8.
doi: 10.1105/tpc.5.9.1081.

Arabidopsis HY8 locus encodes phytochrome A

Affiliations

Arabidopsis HY8 locus encodes phytochrome A

K Dehesh et al. Plant Cell. 1993 Sep.

Abstract

hy8 long hypocotyl mutants of Arabidopsis defective in responsiveness to prolonged far-red light (the so-called "far-red high-irradiance response") are selectively deficient in functional phytochrome A. To define the molecular lesion in these mutants, we sequenced the phytochrome A gene (phyA) in lines carrying one or other of two classes of hy8 alleles. The hy8-1 and hy8-2 mutants that express no detectable phytochrome A each have a single nucleotide change that inserts a translational stop codon in the protein coding sequence. These results establish that phyA resides at the HY8 locus. The hy8-3 mutant that expresses wild-type levels of photochemically active phytochrome A has a glycine-to-glutamate missense mutation at residue 727 in the C-terminal domain of the phyA sequence. Quantitative fluence rate response analysis showed that the mutant phytochrome A molecule produced by hy8-3 exhibited no detectable regulatory activity above that of the phyA-protein-deficient hy8-2 mutant. This result indicates that glycine-727, which is invariant in all sequenced phytochromes, has a function important to the regulatory activity of phytochrome A but not to photoperception.

PubMed Disclaimer

References

    1. Photochem Photobiol. 1992 Nov;56(5):751-8 - PubMed
    1. Plant Cell. 1992 Jan;4(1):29-38 - PubMed
    1. Plant Cell. 1992 Feb;4(2):161-71 - PubMed
    1. Photochem Photobiol. 1989 Apr;49(4):479-83 - PubMed
    1. J Mol Biol. 1982 May 5;157(1):105-32 - PubMed

Publication types

Associated data