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. 1993 Aug;109(4):1024-8.
doi: 10.1111/j.1476-5381.1993.tb13724.x.

Metabolism of endothelin-1 and big endothelin-1 by recombinant neutral endopeptidase EC.3.4.24.11

Z A Abassi  1 , E Golomb, R Bridenbaugh, H R Keiser
Affiliations

Metabolism of endothelin-1 and big endothelin-1 by recombinant neutral endopeptidase EC.3.4.24.11

Z A Abassi et al. Br J Pharmacol. 1993 Aug.

Abstract

1. Inhibitors of neutral endopeptidase EC.3.4.24.11 (NEP) have been shown to attenuate the hypertensive effect of big-endothelin-1 (BET-1) in rats. To determine whether NEP converts BET-1 to endothelin-1 (ET-1), the effect of a recombinant NEP (rNEP) on BET-1 and on ET-1 was assessed in vitro. 2. Incubation of [125I]-ET-1 with 1 microgram ml-1 of rNEP resulted in degradation of the peptide within minutes. Increase in the amount of rNEP to 10 micrograms ml-1 led to total cleavage of [125I]-ET-1 within seconds. 3. Phosphoramidon (10 microM) or SQ-28,603 (100 microM) totally suppressed the degradation of [125I]-ET-1 by rNEP. 4. The degradation of [125I]-BET-1 by either 1 or 10 micrograms ml-1 of rNEP was much slower than that of [125I]-ET-1. Again, both phosphoramidon and SQ 28,603 protected the peptide from degradation. 5. Intact [125I]-ET-1 was not observed when [125I]-BET-1 was incubated with rNEP. 6. These data show that neutral endopeptidase EC.3.4.24.11 is not an endothelin converting enzyme.

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References

    1. J Cardiovasc Pharmacol. 1991;17 Suppl 7:S65-7 - PubMed
    1. Eur J Pharmacol. 1992 Jun 24;217(1):65-70 - PubMed
    1. Hypertension. 1992 Jul;20(1):89-95 - PubMed
    1. J Chromatogr. 1992 Feb 14;574(2):344-8 - PubMed
    1. J Biol Chem. 1992 Jul 15;267(20):14012-8 - PubMed

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