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. 1993 Jun;99(6):457-62.
doi: 10.1007/BF00274098.

A new approach to the immunocytochemistry of cAMP. Initial characterization of antibodies against acrolein-fixed cAMP

J De Vente  1 , J Schipper, H W Steinbusch
Affiliations

A new approach to the immunocytochemistry of cAMP. Initial characterization of antibodies against acrolein-fixed cAMP

J De Vente et al. Histochemistry. 1993 Jun.

Abstract

A method is described to couple cyclic adenosine 3',5' monophosphate (cAMP) to a carrier protein by means of acrolein. Antibodies against this conjugate were raised in mice. These antibodies proved to be highly specific for acrolein-fixed cAMP in a gelatin model system. Slices (300 microns in thickness) from rat cerebral cortex were incubated in vitro and the dopaminergic control of adenylate cyclase activity was drug-manipulated. This manipulation was visualized by application of the cAMP-antisera on cryostat sections of the acrolein fixed slices.

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References

    1. Eur J Neurosci. 1990;2(10):845-862 - PubMed
    1. Neuroscience. 1992 Jul;49(2):479-86 - PubMed
    1. Life Sci. 1984 Dec 3;35(23):2281-96 - PubMed
    1. Neuroscience. 1987 Dec;23(3):823-6 - PubMed
    1. Neuroscience. 1983 May;9(1):23-9 - PubMed

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